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The volume decrease was greatest among people over age 60, but no sex differences were observed

ArticlePubMedPMC 23

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Off-target detection To determine the site-specific cleavage of the CRISPRCas9 system in vivo, the potential off-target loci were searched using Cas-OFFinder ( The selected potential off-target sites were initially PCR-amplified and then subjected to a DNA sequencing assay